Abstract:
Diabetes Mellitus (DM) is one of the most prevalent metabolic disorder that is defined by persistent hyperglycemia condition. Although a variety of therapies are available to treat this disease but all of them have certain side effects therefore scientists are looking for some alternative therapies. Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2 (SHIP-2) is an enzyme that is involved in the hydrolysis of PIP3 back into PIP2. Once activated, SHIP-2 leads to the down- regulation of AKT gene that ultimately disturbs both glucose and lipids homeostasis. Therefore, SHIP-2 is considered as a suitable drug target for the treatment of type 2 DM. The current study was designed to identify a potential phytochemical, derived from the aqueous extract of Thymus serpyllum that could inhibit SHIP-2 protein, thereby ameliorates the blood glucose level. Methodology: Phyto-compounds present in the aqueous extract of T. serpyllum were evaluated for drug-like properties and short-listed compounds were docked with SHIP2 protein to identify a lead compound. This lead compound was then tested in STZ induced diabetic mice model. Results: Out of 376 compounds, only 05 phytochemicals demonstrated good drug-like properties namely; 5-Isopropyl-2-(piperonylidenehydrazino)-2-thiazolin-4-one, gamma.-Lactone of 18- hydroxydeoxycortexone, Coumaran-5,6-diol-3-one, 2-[4-hydroxycarbonylbenzylidene]-, p- Anisyl succinic acid and Phthalic anhydride, 4-benzoyl-. Docking analysis showed that p-Anisyl succinic acid shows binding affinity better than the other four and also better than the commercially available anti-diabetic drugs. Conclusion: The current study shows that p-Anisyl succinic acid is a potential phytochemical that can cure DM, however, further invivo and invitro analysis is required to confirm these findings
