Abstract:
Nanotechnology is the fastest rising technology with great economic outputs due to its usage in a large range of fields including biomedicine, bio-analysis, bio-detectors, drug delivery systems, therapeutics, diagnostics, medical implants analysis, and materials sciences. Iron oxide nanoparticles (IONPs) have gained interest due to a wide range of biomedical applications from probing to therapeutics. In the present study IONPs were synthesized through co-precipitation method by using Iron (III) Chloride Hexahydrate (FeCl3.6H2O) and ferrous sulphate (FeSO4.7H2O) which resulted in the formation of black colored precipitates. IONPs synthesis was further verified using UV-Vis spectroscopy which showed the characteristic peak at the 306nm. X-Ray Diffraction (XRD) was performed to check the crystallinity of the IONPs. Their size and morphology was determined by Scanning Electron Microscopy (SEM) which showed that IONPs are spherical in shape and their sizes are in range of 42-78nm. The FTIR spectrum analysis showed that nanoparticles have hydroxyl group (O-H), alkane group (C-H), carbonyl group (C=C), alcohol group (C-O) and metal and oxygen group (Fe-O) on their surface. MTT assay for cytotoxic analysis of IONPs against MCF-7 and MDA-MB-231 cell lines revealed that they have dose dependent manner of cytotoxicity. As the concentration of IONPs was increased, the cell viability has been decreased. Antimicrobial assay was evaluated against Escherichia coli, Stapylococcus Aureus and Klebsiella pneumonia, which revealed that IONPs have good antibiotic potential against Escherichia coli and Stapylococcus Aureus but have low antibiotic activity against Klebsiella pneumonia. DPPH antioxidant assay showed that IONPs have free radical scavenging activity of 51% at 800g/ml but not significant enough as compare to other standard natural antioxidants like ascorbic acid. These nanoparticles can be used in biomedical application after further optimization such as circulating tumor detection.
