Isolation and Partial Characterization of Phytases Obtained from Botrytis cinerea and Fusarium oxysporum Species for Their Potential Use in Industrial Application

Abstract

Phytases are enzymes utilized as feed additive substance that improve the phosphorus and mineral take-up in monogastric animals and lessen the degree of phosphate discharge in their manure. Fungal extracellular enzymes are easy to cultivate and due to their high production fungi are consider one of best wellsprings of phytase that can be used in the feed business. The present study deals with morphological and ITS based molecular identification of phytase producing plant pathogenic fungal strains. Samples were taken from lab and some samples were collected from area of Gujranwala to get phytases producing fungi on phytase screening media.18S rRNA based sequencing of selected strains was performed and identified as Botrytis cinerea and Fusarium oxysporum. Fungal cultures were subjected to submerged fermentation to find out total units of enzyme produced. Various culture conditions including incubation time, temperature, pH, source of carbon and nitrogen were optimized to get maximum enzyme yield. Maximum enzyme units were achieved with glucose as carbon source, ammonium sulphate and ammonium nitrate as nitrogen source ,6 days of incubation, 25℃ incubation temperature with acidic pH 5 of fermentation media. All strains produce different units Botrytis cinerea strains KST6 and KST51 gave maximum units (31.73U/ml) and (27.36 U/ml) of phytase while Fusarium oxysporum strain NF1 yielded 24.81 U/ml through submerged fermentation under optimized conditions.