Abstract:
Cancer refers to uncontrolled division of abnormal cells and their ability to transfer to sites other
than the origin. Hepatocellular carcinoma is second leading cause of death which arise from
uncontrolled division of abnormal cells in liver that can spread to other parts of body. Viral
induced carcinoma is widespread, and HCV is one of the potent risk factors in HCC. Single
nucleotide polymorphism is widespread phenomenon resulting into mutation in single nucleotide
which leads to genetic changes. There is well established fact that genetics are also involved in
the occurrence of HCC so analysis of various mutations can lead to good diagnostic and
prognostic marker. Protein kinase C is homologous family of protein involved in various cellular
functions. Among 3 types of PKC being novel conventional and atypical PKCZ belongs to
atypical PKC. This research focuses on the implication of PKCZ gene polymorphism by
determining the association of PKCZ (G/A) mutation with liver cancer in Pakistan and
conducting an expression analysis of the polymorphic gene. Samples were collected from liver
cancer patients, followed by DNA extraction and PCR to analyze the allelic and genotypic
frequency, followed by RT PCR for the expression analysis. This study also focuses on in-silico
structural and functional analysis of mutant protein via HOPE an I-Mutant. The results of in vivo
and in silico analysis were aligned with the fact that the heterozygous mutation in PKCZ is
involved in inducing HCC. Whereas the homozygous mutation is more prone to HCV induced
HCC. Molecular docking of antioncogenic molecule known as cangoronine was done which can
be validated by further studies through wet lab.
