Abstract:
Skin is the outermost layer covering 15% of the body and shields it against extrinsic
factors, when get disrupted formed wound. It always remains a challenge for clinician
as skin desecration open a gap for the microbes to invade causing morbidity and
mortality. There are different treatment options available and are already in practice but
limitations restrict their uses. To overcome the limitations, microemulsion-based
hydrogel can be used with Thymus serpyllum and ascorbic acid as active ingredient.
The microemulsion was formulated by using titration method. 40 formulations of
microemulsion were prepared and short listed according to certain criteria, droplet size
and zeta potential was measured as well. Qualitative and quantitative phytochemical
analysis were performed for Thymus serpyllum and bioactive assays including
antioxidant, anti-inflammatory and antimicrobial assays were performed for both plant
extract and selected microemulsion. Skin irritability test was performed to know the
safety of microemulsion for its tropical use. Selected microemulsion was tested for its
ability to heal excision and diabetic wounds. All the results were statistically significant
p<0.05. Microemulsion formed had droplet size 94.95 + 7.1 nm and -14.6 + 0.9 mV
zeta potential. T. serpyllum seed extract contains essential phytochemicals The
microemulsion showed more bioactivity as compared to plant extract. Skin irritability
test was negative for microemulsion as no sign of skin dryness and color change was
observed. The difference was significant for wound healing in different groups.
Microemulsion showed more healing activity as compared to other groups in both
excision and diabetic wounds. Microemulsion healed 100% wounds in diabetic wounds
and 99% excision wounds within 10 days.
