Identification and Isolation of DREB Gene(s) from Solanum lycopersicum

Abstract

Abiotic stressors, such as high temperature, dehydration, and excessive salt concentration reduce crop viability, development, and productivity. Under extreme drought stress condition, tomato growth is restricted which results in low plant yield. To develop plant resistant to different stresses, beneficial functions of DREB genes are studied. Dehydration Responsive Element Bindings are crucial crop drought responsive genes that play a key role in enhancing plant drought tolerance. DREB genes interact with a DRE/CRT cis-element located in the promoter region. DREB genes involve ABA independent pathway to regulate drought in plants. The DREBs play significant roles in the control of plant responses to abiotic stressors. An in-silico analysis is conducted that involved retrieval of tomato DREB sequences and other monocot plants from the kingdom plantae. In order to evaluate the evolutionary relationship and compare the Solanum lycopersicum plant to monocot plants, a phylogenetic analysis is performed using the sequences. Additionally, sub-cellular localization shows that DREB genes both in dicots and monocots have similar localization pattern which indicates their similar functioning in them, but these genes vary in concentration, have higher concentration in dicots than monocots, indicating dicots are better candidates in response to drought stress. In addition to what has already been mentioned, multiscale pocket binding structures of SlDREB with comparison to TaDREB have been discovered which indicated that monocot plants contain more amino acids as compared to dicot plants. Protein analysis shows that dicot peptide lengths are shorter as compared to dicot plants. In wet laboratory techniques, tomato DNA was isolated and amplified using two unique gene primers to identify genes in tomato plants. Prior to sequencing the material, it was purified. Sequences are submitted to the GenBank database