Abstract:
Twenty-two Schiff-bases were synthesized by a condensation reaction in 1:1 M ratios of
thiophene-2-carboxaldehyde with eight different amines and 2-amino thiophenol with
eight different aldehydes in ethanol / methanol solvent. Elemental analysis was used to first
identify the structural determinations of the Schiff-bases, and then UV-Vis, FT-IR, and 1H
NMR were used to confirm them. Good yields of the compounds have been received. The
in-vitro anticancer activity of each synthetic substance was assessed using a human
colorectal cancer cell line (SW480). When compared to Doxorubicin, a reference medicine
with an IC50 value of 32.02 mM, compounds ABSH 11 and ABSH 15 with IC50 values of
0.0826 mM and 8.77 mM demonstrated higher activity, while compound ABSH 07 is
almost as active as Doxorubicin. Different other biological activities such as Antibacterial,
Antifungal, Anti-glycemic, and Anticholinesterase activities of these compounds were also
studied. The compound ABSH 17 is the most potent against Anti-bacterial while ABSH
27 is the most potent against Antifungal activity. ABSH 2 has shown α-amylase inhibition
(56%) as well as anticholinesterase activity (78%) which is in good agreement with
Molecular docking analysis. All other compounds have shown moderate activities.
