Expression and Acetylation Analysis of Adipolin Gene in Type 2 Diabetes Mellitus

Abstract

Type 2 Diabetes Mellitus is a metabolic and multifactorial disorder. It involves insulin resistance which sometimes leads to β cell failure. Adipolin is a novel adipokine released from white adipose tissue. It has insulin-sensitizing and anti-inflammatory effects. The levels of adipolin are found to be reduced in diet-induced obese mice leading to impaired glucose tolerance. The study aimed to check the relative expression and acetylation status of histone H3 Lysine 9 of adipolin in non-diabetics and type 2 diabetes patients. Acetylation of histone H3 Lysine 9 unwinds the chromatin, allowing access of transcription factors to the gene. Adipose tissue biopsies of control and type 2 diabetes patients were collected. Quantitative analysis was done and expression levels were compared in both groups. Acetylation status of histone H3 Lysine was analyzed using chromatin immuno-precipitation protocol followed by gene specific polymerase chain reaction. The expression of adipolin was found to be reduced in type 2 diabetes patients as compared to controls. Both groups were found to have acetylated histone H3 Lysine 9 in adipolin gene region. So it was concluded that type 2 diabetes patients have suppressed expression of adipolin but this was not due to de-acetylation of histone H3Lysine 9 in adipolin region. Further studies are required to find the causative factor of adipolin down regulation.