Synthesis and Characterization of Antibacterial Bilayer Hydrogel for Wound Dressing Application

Abstract

Nanoparticles are frequently employed in biological applications, medicine delivery, and water treatment. The concept of incorporation silver nanoparticles (AgNPs) in membranes is useful for wound dressing and covering surgical instruments because the metal base nanoparticles are the intriguing material for wound healing, antibacterial and drug carriers. A lot of study has been done in recent years on the slow release of drugs. Many researchers have tried how to accelerate the wound healing. Some authors have tried hybrid hydrogel by using different polymers to give stability and drug release properties. But they have not mentioned any data regarding the quantity of the drug that is released around the wound area and how much the drug should release either it releases fast or slow and which one gives the better results. Although silver has good antibacterial properties, but they have not mentioned any side effects if we increase the concentration around the wound area. They haven't mentioned the systemic toxicity of the silver because it varies in different application. We are using different sizes of AgNPs in bilayer hydrogel membrane their shape is spherical and quantity of AgNPs is constant to see its antibacterial and toxicity test for wound dressing. Silver nanoparticles was characterized by UV Vis, Zetasizer, SEM Apreo, S(T)EM, XRD and FTIR. After analyzing the AgNPs we have incorporated these AgNPs in bilayer hydrogel membrane. Bilayer hydrogel membrane was characterized by SEM Apreo, XRD, TGA, AFM. Some physical testing was also performed on membranes like swelling test, moisture retention and Water vapor transmission rate. The antibacterial activities were conducted against E. coli and the maximum inhibition zone was reported 20±5nm. MTT assay test was conducted to see cell viability % and we observe that at low concentration we get the best results between 85% to 92.2%. Future research and development would benefit from both in vivo and in vitro methods to extrapolate from and test antibiotic-loaded nanofibers in humans.