Detection and cloning of P1 gene of Sugarcane mosaic virus

Abstract

Sugarcane is the main cash crop of the Pakistan. Pakistan is the seventh largest Sugarcane producing country. Major loss of sugarcane yield is due to Sugarcane mosaic virus which belongs to family potyvirid, largest family of plant viruses containing single stranded positive sense RNA.Three major proteases are included in the genome of the SCMV i.e. P1, Hc Pro, N1a, P2-1 helper component. P1 is the major non conservative protein of the SCMV. Non-conservative nature of the P1 gene of the sugarcane is responsible for determining host range of the sugarcane. The Identification and cloning of the P1 gene of the Sugarcane mosaic virus will be helpful for screening of the infected sugarcane cultivars of the Pakistan.