In silico Analysis of Cytochrome b5 Gene Family in Triticum aestivum

Abstract

Abruptly altering climatic patterns pose serious threatsto the viability of plants which needs to be catered to ensure survival and sufficient yield of plants. The multiple abiotic stressors playing havoc include heat stress, water deficit, chilling, and saline stress. Various plant protein families perform defensive roles against the stresses experienced by plants. In current research work, wheat cytochrome b5 (TaCYB5) ubiquitous protein gene family was subjected to identification and characterization. Multiple in silico analysis including identification of sequences, motifs, gene structure, amino acid composition and chromosomal mapping were performed for the scrutiny of reported sequences. This analysis revealed the significance of domain architecture in multiple stress catering functions of TaCYB5 gene family. Functionality of TaCYB5 genes was explored via gene ontology, cis-regulatory elements and subcellular localization approaches indicating multiple stress-related responses. Moreover, in silico transcriptome analysis, post translational modifications, protein-protein interactions, protein modelling and protein protein (TaCYB5 - NADH CYB5-Reductase) docking revealed the essential amino acids involved in enzymatic catalytic reactions. Current work reported a total of TaCYB5 genes on ABD genome. Expressional analysis and computerized simulations of the docked complex may reveal biological pathways and functionality of the reported unknown protein interactors which may be helpful in producing stress resistant crop cultivars producing sufficient yield and acting as a booster shot for current economy.