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Ultrasound-Mediated Enhanced Drug Delivery in Glioblastoma Cell Line Using Peptide-Tagged Microbubbles

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dc.contributor.author Fatima,Mushkbar
dc.date.accessioned 2023-06-13T11:12:52Z
dc.date.available 2023-06-13T11:12:52Z
dc.date.issued 2023
dc.identifier 327753
dc.identifier.uri http://10.250.8.41:8080/xmlui/handle/123456789/33979
dc.description Supervisor : Dr. Shah Rukh Abbas
dc.description.abstract Ultrasound as a non-invasive and non-ionizing technology has been utilized as an efficient technique for the transport of drugs and genes in addition to its use of imaging. Sonoporation (cavitation generation by microbubbles), one of the applications of ultrasound contrast agents has been utilized as an effective approach for the opening of the blood-brain barrier. In combination with the targeted microbubbles, these act as effective delivery vehicles for the transport of anti tumor drugs to enhance drug uptake and enhance the treatment of Glioblastoma multiforme. In this study, we have designed gH625-tagged microbubbles which can cross the blood-brain barrier by adsorption mechanism. These microbubbles were synthesized by the solvent displacement method and were linked to the membrane crossing peptide (gH625). Two types of microbubbles were synthesized: core-containing Cisplatin microbubbles and shell-encapsulating Amygdalin microbubbles, as microbubbles had core and shell pockets for drug loading. These pre-PGS, gH625-tagged microbubbles were characterized through FTIR and SEM analysis. Microbubbles were taken by the glial cells one of the components of BBB and have been able to be uptake by the U-87 cell line (a glioblastoma multiforme cell line). Cell viability was analyzed and compared with the bare drug. Upon ultrasound exposure, the overall cytotoxic activity of anti-tumor drugs loaded pre-PGS microbubbles alleviated as cavitation nuclei enhanced the uptake of the drug while reducing the systemic side effects. Thus, ultrasound-mediated gH625 tagged pre-PGS microbubbles not only helps to open the BBB by generating reversible gaps in the endothelial layer of the BBB but also enhance the anti-neoplastic activity in addition to the safe delivery carrier function of the microbubbles, minimizing the side effects of the drugs en_US
dc.language.iso en en_US
dc.publisher Atta Ur Rahman School of Applied Biosciences (ASAB), NUST en_US
dc.subject Ultrasound, Drug, Glioblastoma, Peptide, Tagged, Microbubbles en_US
dc.title Ultrasound-Mediated Enhanced Drug Delivery in Glioblastoma Cell Line Using Peptide-Tagged Microbubbles en_US
dc.type Thesis en_US


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