Abstract:
Protein kinase θ is a novel isozyme comprising 706 amino acids. The role of protein kinases theta
has been explored in several different cancers like breast cancer, leukemia, gastrointestinal stromal
tumor and other cancers. The structure of KPCQ was determined, validated and domains were
analyzed through computational approaches. In-silico methodology was adopted for carrying out
protein-protein docking and determining the hydrophobic as well as hydrophilic interactions
amongst them. This particular study aims to investigate the possible role of KPCQ , p21 and c-jun
in the progression of breast cancer. Two-hundred samples were analyzed by real-time quantitative
PCR for determining the relative expression of KPCQ, p21 and c-jun in breast cancer patients
(stage I, II, III, IV). The target gene and interacting protein expression was also linked with the
risk factors as well as clinical features of breast cancer. The study concluded that the expression
of PRKCQ and c-jun was elevated in breast cancer patients. However, the tumor suppressor gene,
p-21, expression was downregulated. The genes showed differential expression when linked to
risk factors and clinical features. Lastly, molecular dynamic simulations provided evidence related
to the structural and functional changes due to the induced mutation. All this information is useful
for early diagnosis and developing therapeutics for treatment of cancer.
