Abstract:
Breast cancer is a leading cause of mortality among women worldwide, and while numerous
biomarkers have been identified for detection and treatment, there is a continuous need for
novel and more efficacious biomarkers. Kinase Protein C Zeta (KPCZ) is a protein associated
with cancer development and has been implicated in multiple cancer signaling pathways. This
study aims to investigate the pathogenicity of KPCZ in human breast cancer by analyzing
single nucleotide polymorphisms (SNPs), predicting its three-dimensional structure, examining
sub-cellular localization, and assessing interactions with other proteins. By analyzing
genotyping data from breast cancer patients and healthy individuals, the study identifies the
association of pathogenic non-synonymous (rs367917640) KPCZ SNPs with breast cancer
where homozygous AA genotype showed association with breast cancer with its highest
frequency distribution in patient samples. Furthermore, this study examines the trends in
relative expression of KPCZ, EGF, and NFkB biomarkers, as well as their correlation with
various clinical features and risk factors associated with breast cancer. It reveals that KPCZ
plays a significant role in cancer development, with its overexpression is linked to breast
carcinogenesis. MD Simulations revealed that docking structure of G65A mutated complex
with PDK1 showed a more stable confirmation as compared to the wildtype KPCZ complex
