Abstract:
Alzheimer's disease (AD), the most common progressive neurodegenerative illness in older
individuals, is characterized by declining cognitive ability. Cognitive decline and memory loss
are two of the first pathological signs of Alzheimer's disease by many factors including oxidative
brain damage. Several studies have reported improvement in memory and cognition impairment
by the consumption of high doses of Limosilactobacillus strains and phytochemicals like
Quercetin. Through promoting the growth of the HPA axis and the manufacture of
neuromodulators such antioxidant enzymes, GABA, SCFAs, serotonin, and BDNF, the gut
microbiota influences the gut-brain axis. The current study's objective is to investigate the anti Alzheimer’s effect of potential Limosilactobacillus fermentum Y55 (1.5x109 CFU) and
Quercetin (25mg/kg) in combination on AlCl3- induced AD rat models. Rats were assigned to six
distinct groups at random (n = 6). Using a variety of behavioral tests, including the Elevated Plus
Maze (EPM), New Object Recognition (NOR), Y-maze, and Morris Water Maze, researchers
investigated the impact of this combination on anxiety and memory (MWM) to determine
whether recovery plays a part in reducing Al's neurotoxic effects. Male rats were exposed to
aluminum along with Limosilactobacillus fermentum Y55 and Quercetin mixed in distilled water
and after completion of treatment their learning and memory was tested using Novel object
recognition test, y maze test and Morris water test paradigms and collated with the controls. To
assess the anxiety, an elevate plus maze test was performed. The animals from treated and their
controls were euthanized and dissected after the behavioral tests and the brains were removed to
extract hippocampus for further histological analysis. The results unfolded those rats treated with
probiotic and polyphenol combination had exhibited significantly improved working, reference
memory, recognition and spatial memory with reduced anxious behaviors. Histologically, this
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combination has improved the normal architecture of the brain hippocampus in TPQ group. H
&E staining showed that TPQ group had normal morphological features with proper round and
intact cell bodies as compared to DC group. To cap it all, this study demonstrates that animals
exposed to Limosilactobacillus fermentum Y55 and quercetin together show marked
improvement in their cognitive functions and memory and improves the morphology of the main
targeted parts of brain that is hippocampus.
