Study the association of polymorphisms and high thorough-put analysis of PTEN and XRCC1 genes with HCC risks in patients suffering from viral and non-viral Hepatocellular Carcinoma.

Abstract

Hepatic cirrhosis and Hepatocellular carcinoma (HCC) significantly contribute to the global disease burden and mortality rates. Amid the diversity of characteristics intricate the expanding frequency of cancer of the liver cells, its late diagnosis and difficulty in discrepancy flanked by late-stage cirrhosis from initial stages of HCC are the major ones. It necessitates facilitating diagnostic tests for earlier diagnosis of HCC and the ability to predict its progression from underlying cirrhosis. Several blood-based biomarkers and single nucleotide polymorphisms of many genes have been proposed to detect HCC at its early stages in recent years, such as AFP is an already reported blood-based biomarker. DNA from blood samples of 150 patients and 50 controls was extracted and processed by using SNP specified polymerase chain reaction then sequencing PCR to perform SNP specified sequencing. Analysis of sequencing data showed that the single nucleotide polymorphism of PTEN (rs1234220) and XRCC1(codon280) is present in the study samples. In case of PTEN (rs1234220), normal genotype AA was prevalent among controls whereas AG genotype was prevalent among patient samples. Analysis of sequencing data of XRCC1 showed that there was no normal genotype present, all samples including healthy controls have mutated forms among them AG was more prevalent in all samples whereas TG and then GG was less prevalent. It gives the idea that if these SNPs are detected earlier, the prognosis of HCC will be improved as both of them are reported to be associated with higher risks of cancer of liver cells