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Use of qPCR Analysis for the Diagnosis and Surveillance of Pathogens in Pakistan

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dc.contributor.author Tariq, Namra
dc.date.accessioned 2023-09-28T06:33:15Z
dc.date.available 2023-09-28T06:33:15Z
dc.date.issued 2023
dc.identifier.issn 00000318597
dc.identifier.uri http://10.250.8.41:8080/xmlui/handle/123456789/39348
dc.description Dr Imran Hashmi en_US
dc.description.abstract Water is a crucial component for sustaining life on planet earth and the availability of clean and safe drinking water has become a huge challenge in the present era. Rawal Dam is an important source of drinking water within the twin cities of Islamabad and Rawalpindi, Pakistan. This dam is supplied by natural freshwater from various natural streams and rivers. Some of the important tributaries of Rawal dam include Nurpur stream, Shahdara stream, Jinnah stream, Korang River and Jillani Town stream etc. As these streams are located close to the residential and industrial areas in Islamabad, Pakistan, so they are continuously being polluted by the anthropogenic activities. Due to the waste intrusion within these freshwater bodies, the water quality is highly being compromised and as a result the health of the people residing close to them is in danger. The present study focuses upon the water quality assessment of Rawal dam and its tributaries. The water quality of the selected sites has been assessed by physiochemical tests, microbial analysis, molecular analysis, (DNA extraction and qPCR analysis) and heavy metal analysis. This study will be helpful in the identification and quantification of notorious Gastroenteric pathogens including Salmonella, Shigella, E. coli and Campylobacter within the water bodies and will be helpful in tracking down the transmission routes of Gastroenteritis in Pakistan. en_US
dc.language.iso en en_US
dc.publisher Nust, IESE en_US
dc.title Use of qPCR Analysis for the Diagnosis and Surveillance of Pathogens in Pakistan en_US
dc.type Thesis en_US


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