COMPARATIVE GENOMIC ANALYSIS OF PANTOEA ANANATIS NCCP568 TO STUDY ITS GENOMIC DIVERSITY AND ANTIBIOTIC RESISTANCE

Abstract

Abstract

Pantoea ananatis is gram-negative bacteria of rod-shape, facultative anaerobic nature and member of the Erwiniaceae family of the Enterobacteriaceae group. In addition to occurring endophytically in hosts where it has been documented to induce signs of disease as well as in species where no such symptoms have been detected, it is mostly epiphytic in nature. Its Ubiquitous nature makes it biotechnologically and environmentally significant. In order to better understand the genomic diversity and antibiotic resistance a local P. ananatis strain NCCP 568 was sequenced using universal 16S rRNA sequencing primers at Macrogen, Korea (Seoul, Korea). The genomic size of the strain NCCP 568 is 4.685 Mbp with 53.49% GC content, 0 plasmids and 21 contigs. The genome contains 4764 protein coding genes and 68 non-coding RNA genes. The closely related P. ananatis strains include PA13 which is representative strain from Korea, JT1-188, R100, JT8-6, YJ76, NN08200, JBR-LB3-16, FDAARGOS_680, AJ13355, LMG 5342 and TZ39. Several resistant genes were identified in P. ananatis NCCP568 including vanG, ArnT, PBP3, gyrB, CRP, rsmA, KpnH and adeF

genes. These genes are known for conferring resistance aagainst vancomycin, colistin, beta- lactam antibiotics, fluoroquinolones, erythromycin, cloxacillin, oxacillin, norfloxacin,

azithromycin, imipenem, polymyxin and tetracycline. Comparative analysis of NCCP568 strain with other complete genome sequence strains revealed that the local strain NCCP568 is more resistant and virulent compare to other complete genomes. Phylogenetic analyses inferred that NCCP568 is closely related to FDAARGOS, JT1-188, TZ39, JT8-6 and Lstri and distantly related to PA13 reference strain.