POSSIBLE CROSS TALK BETWEEN TPD52, KLF13, MIR-223 AND PKCƐ IN PROSTATE CANCER

Abstract

Prostate cancer is the second most common cancer in man. In Pakistan annual mortality rate of prostate cancer is estimated at 0.9/100,000 people and rising rapidly. Many molecular mechanisms involved in onset and progression of prostate cancer still remains unclear. The aim of the present study is to investigate the possible cross talk between TPD52, KLF13,PKCɛ and miRNA-223 and to find out the three dimensional structure of KLF13. 50 blood samples of prostate cancer patients were collected from combined military hospital Rawalpindi (CMH) and expression analysis was performed using Real Time PCR. The Fold change was calculated through delta cycle threshold method. The statistical significance was calculated through one way and two ways ANOVA. The expression of TPD52 is highly elevated i.e. 22 fold increased as compared to healthy control while expression of miR-223, PKCɛ and KLF13 is lower i.e. 0.0168 fold decreased as compared to healthy controls. Expression of TPD52, KLF13, PKCɛ and miR-223 was also analyzed according to stage, grade, and metastasis and treated as well as non- treated patients. In the end we also find out the possible cross talk between all genes and it was found that all genes are linked with kras and AKT pathway. Further study will help in the development of new diagnostic marker and will provide new insights into the potential mechanisms of oncogenesiss and metastasis of Prostate cancer.