Intricate Role of KLF16 Variants in Breast Cancer Pathogenesis

Abstract

Breast cancer is prevailing all over the world at a very fast pace and now become the leading cause of death among women. Poor prognosis, reoccurrence of breast cancer, and resistance to current therapies can cause high mortality rates in infected women therefore this study aims to identify a novel KLF16 genetic variant to improve diagnostic accuracy, prognosis prediction, and development of targeted therapies for breast cancer. KLF16 is a gene involved in multiple cancer signaling pathways and causes cancer progression. In-silico analysis was performed in which the three-dimensional structure of the protein was validated and the effect of a variant of the protein’s structure and functions were studied. In experimental analysis, the genotypic data from breast cancer patients and healthy individuals was retrieved, and their association with the KLF16 missense variant rs761994763 was analyzed. The CC genotype has the highest frequency distribution in samples of patients indicating its potential link with breast cancer. This study helps to identify novel KLF16 variants, better understand the effect of the variant on the structure and function of the KLF16 protein, and uncover the association between the KLF16 variant and breast cancer in Pakistan.