Evaluation of Stilbenes biosynthetic gene(s) upon hormonal treatment in Arachis hypogaea

Abstract

Peanuts ( Arachis hypogaea), also called ground nut are world’s vital oil producing crop. They are consumed all over the world due their high nutritional value. Peanuts contain many compounds like proteins, fibers, polyphenols, antioxidants, vitamins and minerals. In recent times, peanuts are getting higher attention due to their containment of resveratrol and stilbenoids in the kernels and other parts of plant. Stilbenes and stilbenoids are a well known class of naturally occurring small group of phenylpropanoids which have been identified in at least 72 unrelated plant species. These compounds are stress metabolites produced in leaves and other parts of the plant in response to various biotic and abiotic stresses stresses such as infection, wounding, UV-C exposure and treatment with chemicals. In this study, Structural and functional analysis of stilbenes synthase gene (s) was performed through In-silico analysis. In addition, Real Time PCR evaluated the expression of putative gene upon hormonal treatment in two registered varieties (Golden and BARD) of peanut plant. Differential expression was observed in both the varieties. However, Pattern of expression of putative genes was observed to be different between both the varieties (Inter-Varietal differential expression). The anaylsis showed that hormonal treatment evidently triggers Stilbenes production in Arachis hypogaea.