Abstract:
The immune framework of human body embarks flexible natural defense constituting a
family of cells and molecules that have ability to recognize mild chemical differences to
foreign invading pathogens from one another and host’s own proteins and cells. The
diversity of immune system overtime sparks the need to explore the underlying
mechanisms for a systematic understanding. Family with sequence similarity 26, member
F (FAM26F) is a recently identified molecule in the quest for control of lethal diseases as
immune regulator, whose modulatory pathways are yet to be investigated. It has been
reported to involve in differential expression in infections, contact among dendritic and
NK cells, cytotoxicity and activation of NK cells, and induction of interferon gamma and
associated genes. So, the present study was designed to analyze the cytokine induced
stimulatory pathways of FAM26F in human peripheral blood mononuclear cells. IFN-α,
IFN-γ and TNF-α were selected as immune modulators playing fundamental part in inborn
immunity. Real time PCR analysis showed significant upregulation of FAM26F by IFN-α
followed by mild increase in expression by IFN-γ and TNF-α. The results suggest positive
correlation of FAM26F with IFN-α stimulated NFκB pathway highlighting that FAM26F
is induced downstream by NFκB. The findings uphold the position of FAM26F in innate
immunity. In future, there is a need to further investigate FAM26F role in clinical settings
through mRNA expression levels and proteome analysis.
