In-vitro-sonothrombolysis by using streptokinase loaded pre-PGS microbubbles for the treatment of Ischemic stroke

Abstract

Microbubble-mediated sono-thrombolysis is an effective method of thrombus breakdown. The goal of this Invitro study was to determine the efficacy of Invitro sonothrombolysis by using streptokinase-loaded pre-PGS microbubbles. Synthesis of streptokinase loaded microbubbles was carried out by using solvent displacement method and synthesis was confirmed by FTIR and SEM analysis. Invitro blood clot models synthesized by using human blood were subjected to 30 minutes of sonothrombolysis in study groups of US alone, US+MB, US + Drug and US+ Drug loaded Microbubbles. Frequencies used for this purpose were 2.8 MHz and 3.2 MHZ at a Mechanical index of 0.1, 0.3, 0.6 and 0.9. Outcomes of sonothrombolysis were monitored by measuring pre and post weight of clot, mean gray intensity analysis of images captured during 30 minutes of sonothrombolysis by using image. J, histological analysis of clot residues in which fibrin degradation was assessed and spectrophotometric absorbance of hemoglobin present in effluents of blood clots collected after 30 minutes of the procedure. Monitoring outcomes depicted that maximum reduction in mean gray intensity and maximum degradation of fibrin network, and higher spectrophotometric absorption took place by using drug loaded microbubbles in the presence of ultrasound. This Invitro sonothrombolysis by using drug-loaded microbubbles has shown promising results and can be further utilized for evaluating its potential in in vivo studies.