Abstract:
Acinetobacter baumannii is an opportunistic pathogen that causes various hospital and
community-acquired infections. According to WHO, it is a priority one critical pathogen
and poses a potential hazard to human health. Thus, the goal of our study was to develop
techniques for detecting Acinetobacter baumannii, determining its antibiotic susceptibility
pattern, and providing a therapeutic alternative to eliminate antimicrobial resistance.
In our investigation, we gathered two types of samples: clinical and hospital wastewater.
Clinical samples were utilized to diagnose Acinetobacter baumannii and determine its
antibiotic resistance pattern. Bacteriophage was isolated from wastewater, and its lytic
activity was tested using a spot test and plaque assay.
Antibiotic susceptibility testing determined that Amikacin, Cefepime, Gentamicin,
Meropenem, Piperacillin-tazobactam, Imipenem, and Levofloxacin, are resistant to
Acinetobacter baumannii, only effective drug is Doxycycline that showed 21% resistant.
And the isolated phage has significant lytic activity against the A. baumannii strain DSM
30007. Thus, our investigation includes diagnostic procedures, antibiotic susceptibility
patterns, and phage therapy for Acinetobacter baumannii clinical strains.
