Abstract:
Microalgae are gaining significant interest due to their intracellular components, including high-
value phycobiliproteins (PBPs). PBPs are natural pigment-protein complexes with antioxidant and
anti-inflammatory properties, making them beneficial for the food, nutraceutical, cosmetic, and
biotechnology industries. However, large-scale commercial exploitation remains hindered by the
high cost associated with PBP extraction from microalgal biomass. While the freeze-thaw method
has been reported as an effective extraction technique, further optimization is crucial for industrial
viability. This study investigates the efficiency of the freeze-thaw method for PBP extraction from
Nostoc sp. cultivated in a novel lab-scale raceway pond system. This research aims to evaluate
the impact of this cultivation method on microalgal yield compared to conventional techniques.
The findings will contribute to the development of cost-effective and sustainable strategies for
large-scale PBP production from microalgae
