Correlation among KLF14, Tumor Protein D52, PKCε and Micro RNA 124; Brain Cancer

Abstract

Brain cancer continues to be the cause of high level of mortality worldwide. Most common form of gliomas in humans are astrocytoma, oligodendroglioma and glioblastoma multiform. Early diagnosis of these cancers is vital for successful treatment. Proteomics may play a crucial role for early detection and diagnosis that lead toward the discovery of biomarker. The aim of the current study is to investigate the possible relation of TPD52, KLF14, miR-124 as well as PKC epsilon in the progression and development of brain cancer in addition to Space occupying lesion of brain. Sixty human blood samples comprising varying diagnostic groups (SOL brain, grade I, grade II, grade III, grade IV) were run to find out the expression of these genes using delta cycle threshold method of Real Time PCR. The statistical analysis was performed through one-way and two- way Anova. TPD52 and PKC epsilon were upregulated in brain cancer by 2.5 and 1.6 fold respectively in comparison to healthy controls. KLF14 and miR-124 found to be downregulated in brain cancer by 0.5 and 0.002 fold respectively. In the current study, expression of TPD52, KLF14, and miR-124 as well as PKC epsilon with metastasis, tumor stage and gender in the patients of brain was observed. An elevated expression of TPD52 and PKC epsilon in the blood of brain cancer patients, while the downregulation of KLF14 and miR-124 indicate the metastasis and advanced stage of tumor. In the current study, these proteins found to be differentially expressed in the blood of space occupying lesions (SOL). Upregulation of TPD52 and PKC epsilon however, reduced expression of KLF14 and miR-124 in SOL of brain as compared to healthy controls was observed. Above differentially expressed proteins provide useful information on the differences existing between normal brain, SOL in addition to gliomas; thus might prove to be useful having diagnostic or prognostic value.