Abstract:
Nosocomial Infection, commonly known as Hospital-Acquired Infection (HAI), is caused by viral and bacterial pathogens in hospitals and other clinical settings. This is a main problem and is responsible for growing number of deaths along high medical costs. Different strategies have been established to reduce the frequency of nosocomial infections. One way to avert contagion is by modifying surgical blade and surgical fabric’s in a way that no bacterial adhesion can occur. In this study, silver nanoparticles were biologically synthesized from plant extract Aerva javanica. Silver Nanoparticles along with PDAC were deposited layer by layer on fabric and electrochemically on surgical blade. The morphology and conformation of the coatings had been studied using scanning electron microscopy (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Energy dispersive X-ray (EDX) was also used to confirm the presence of silver nanoparticles within the coated samples. The coatings had been tested against hospital oriented bacterial species (Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, Klebsiella pneumonia, Methicillin-resistant Staphylococcus aureus and Enterococcus feacalis) to observe their antibacterial properties. The antibiofilm activity of silver nanoparticles coated blade and fabric was tested to bacterial specie pseudomonas aeruginosa and shows clear distortion and bacterial inhibition. The stability of silver nanoparticle coating on surface of fabric and blade was confirmed through release profile mechanism. Furthermore, for industrial concern silver nanoparticle coated fabric was checked for its washing durability. In conclusion, nosocomial infections are prevented to a great extent by the use of silver nanoparticles coated blades and fabrics.
