Abstract:
Hepatocellular carcinoma is a leading cause of cancer-related deaths due to its
complexity in diagnosis, chemo-resistance, and aggressive nature. Identification of
pathogenic SNP in PKCι can be a potential biomarker in early diagnosis and treatment
of HCC. In this study high risk variants of PKCι associated with HCV induced HCC
were identified and validated in the wet lab. The association of viral pathogenicity
was checked with the identified potentially pathogenic variant of PKCι. The in silico
structural and functional analysis of the selected SNP of PKCι was carried out using
different online tools. Molecular dynamic simulations were carried out for further
confirmation. Last of all, the analysis of molecular interaction of PKCι with potential
inhibitor, Decahydrocyclopentachrysenediol, was performed to assess its therapeutic
potential. The identified pathogenic SNP (rs ID1199530606) of PKCι showed
significant association with hepatocellular carcinoma. So, it may be used for prognosis
of hepatocellular carcinoma. Glycine changes into tryptophan at identified variant (rs
ID1199530606) position and caused the overall stability of PKCι to decrease.
Docking results of Decahydrocyclopentachrysenediol with PKCι revealed that
Decahydrocyclopentachrysenediol can be a potential inhibitor of PKC
