Investigating Pathogenic SNPs of PKCι in HCV Induced Hepatocellular Carcinoma

Abstract

Hepatocellular carcinoma is a leading cause of cancer-related deaths due to its complexity in diagnosis, chemo-resistance, and aggressive nature. Identification of pathogenic SNP in PKCι can be a potential biomarker in early diagnosis and treatment of HCC. In this study high risk variants of PKCι associated with HCV induced HCC were identified and validated in the wet lab. The association of viral pathogenicity was checked with the identified potentially pathogenic variant of PKCι. The in silico structural and functional analysis of the selected SNP of PKCι was carried out using different online tools. Molecular dynamic simulations were carried out for further confirmation. Last of all, the analysis of molecular interaction of PKCι with potential inhibitor, Decahydrocyclopentachrysenediol, was performed to assess its therapeutic potential. The identified pathogenic SNP (rs ID1199530606) of PKCι showed significant association with hepatocellular carcinoma. So, it may be used for prognosis of hepatocellular carcinoma. Glycine changes into tryptophan at identified variant (rs ID1199530606) position and caused the overall stability of PKCι to decrease. Docking results of Decahydrocyclopentachrysenediol with PKCι revealed that Decahydrocyclopentachrysenediol can be a potential inhibitor of PKC