Abstract:
Rheumatoid arthritis is a progressive auto-immune disease in which body immune system attacks
its own self tissues especially the synovial lining of the joints. There is no cure for R.A. Therapeutic
strategies mainly focus on the symptoms and managements. The current therapies lead to the
increase cytotoxicity and many other side effects mostly gastrointestinal bleeding and peptic
ulcers. So, a new approach is required for anti-inflammatory and anti- rheumatic potential with
minimum side effects. Medicinal plants promise these results with minimum side-effects as
phytochemicals are natural compounds, they are less likely to induce cytotoxicity. For comparative
evaluation of Cassia Angustifolia and Colchicum Luteum qualitative and quantitative
phytochemical analysis was done to check the presence of medically important components and
the phenolic and flavonoid content. Anti-oxidant potential was analyzed by DPPH and H2O2
scavenging assay whereas anti-inflammatory activity was determined by membrane stabilization
assay and albumin denaturation assay. Safety of extract was determined by Eneguide acute toxicity
analysis after which CIA mice mode was made for the comparative evaluation of anti-rheumatic
potential of Cassia and Colchicum. For the identification of lead compounds responsible for the
anti-rheumatic potential GCMS was conducted. The active compounds were then isolated from
the GCMS data on the basis of Druglikliness, AMES, Lipinski rile of 5, BBB impermeability and
rotatable bonds. Shortlisted compounds were then docked with the HUB genes isolated from the
DEGs after the PPI generation. The four HUB genes were selected i.e. STAT1, UBC, EEF1A1
and RPL19. The docked complexes with the highest binding energies were then validated by MD
simulations. Colchicum Luteum shows much promising results as compared with the Cassia
Angustifolia as it shows higher flavonoid and phenolic content and better anti-oxidant and anti inflammatory properties. Moreover, Colchicum CIA mice swallowing diminished at the elevated
rate than that of Cassia. Colchicum compounds also shows much higher docking energies than that of Cassia. Therefore, Colchicum Luteum has much stronger anti-oxidant, anti-inflammatory and anti-rheumatic potential. The novel compounds which shows stable binding during the simulations should be selected for the drug development of the rheumatoid arthritis
