Antibacterial activity of dental composite integrated with ciprofloxacin loaded silver nano particles

Abstract

Resin composites have been widely used in dental restoration. However, polymerization shrinkage and resultant bacterial microleakage is a major limitation that may leads to secondary caries. To overcome this, a new type of antibacterial resin composites containing ciprofloxacin loaded silver nanoparticles were synthesized. Ciprofloxacin loaded silver nanoparticles were successfully synthesized with chemical reduction method which was confirmed by Ultraviolet–visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and Zeta potential. Ciprofloxacin loaded silver nanoparticles were added into resin composites. The antibacterial properties of these ciprofloxacin loaded silver nanoparticles modified resin composites against Enterococcus faecalis, Streptococcus mutans and Saliva Microcosm model were evaluated. Cytotoxicity of these modified resin composites was determined by performing hemolytic assay. Compressive strength (CS) of these modified resin composites was assessed by a universal testing machine. The results indicated that the antibacterial activity and compressive strength of resin composites containing Ciprofloxacin loaded silver nanoparticles were superior to the control group and also exhibited less cytotoxicity as compared to the resin composites containing silver nanoparticles. In short, these results established strong ground applications for CIP-AgNPs modified dental composite resins.