Abstract:
The present study reports a green, cost effective and biological methodology
to synthesis Titania nanoparticles from algal extracts of Dictyosphaerium sp. Strain
HM1 (DHM1). This synthesis method was also optimized by different
physiochemical parameters like temperature, reaction time, molar concentration and
calcination temperature to obtain different crystalline form of TiO2 nanoparticles. The
results were initially characterized by UV-VIS spectrophotometry showing maximum
absorbance at 450nm and information regarding size distribution and morphology was
visualized through SEM which ranges from 30-50 nm. EDX analysis confirmed the
elemental analysis of TiO2 NP‟s and XRD confirmed the different crystalline forms of
TiO2 NP‟s (Anatase and Rutile). These TiO2 NP‟s were than doped with gold and
silver to obtain AuNP‟s and AgNP‟s in order to check its antibacterial, antibiofilm
and anticancer activity. It was concluded than undoped TiO2 had no pathogenic
activity while doped nanoparticles showed pathogenic behavior with AuTiO2 NP‟s
having highest antibacterial and antibiofilm activity. Moreover, results from invitro
toxicity suggest very exceptional anticancer activity of AuTiO2 NP‟S against
colorectal cell line HCT116 and the triple negative human breast cancer cell lines
HCC1954. This healing can also induced marked apoptosis leading to typical cell
cycle arrest in G2/M phase. It is recommended to further characterize these
nanoparticles by using advance characterization techniques like NMR, Raman
spectroscopy and TEM analysis. In silico approach should be adapted to examine the
interaction between doped nanoparticles and their cellular interaction with bacteria and
human cell.
