Experimental Study of Multi-cell Counting Based on Inertial Microfluidic Devices.

Abstract

In the field of biomedical, a cell counting device is used for the counting of cells or microparticles for the treatment of different kinds of diseases. The research based on the inertial flow microfluidic focused cell counting. The aim of study is to design and development of a low-cost microfluidic counting device for counting of particles in a focused microchannel. The counting of particles is independent of size, device can be count for different sizes. Using fluorescent microscope and AM Scope the image-based counting has been done at different sized microchannels. Which were fabricated using 3d printed mold and PDMS pouring on the mold. PDMS and Glass slide is bonded using plasma bonding. In the research work, comparison between two scopes have been verified at two different particles solution ratio. The image-based counting has been done by taking videos from the scope’s cameras. The particles counting validated by counting the particles using a commercial device Hemocytometer. Results from both the scopes are mainly same, the counting from any of scope can be done and it can be done on mobile phone in future as the results are almost same for both the cases. There were three channel sizes and four different flow rates on which the experiment performed. The experiment performed in a clean room, which shows that the device having least dimension is best for counting microparticles. Also, the counting depends on the flow rate of the solution because increasing the flow rate decrease in total count of microparticles. It can used in lab tests for medical diagnostics, can be portable, easy to use and a low-cost device.