SYNTHESIS,CHARACTERIZATION AND APPLICATION OF NATURAL NANO COMPOSITES

Abstract

The use of natural polymers in the creation of skin wound dressings has gained attention for its potential to speed up healing and protect against pathogens. In this study, the ability of the Papaver somniferum plant to heal wounds in various forms, such as pure plant extract, plant-derived silver nanoparticles, and their inclusion in a polyvinyl alcohol hydrogel, was investigated. Characterization of the compounds was done using UV spectrophotometry, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersive spectroscopy. Antimicrobial tests showed that silver nanoparticles had a zone of inhibition of 12mm against Staphylococcus aureus, while the silver nanoparticle hydrogel had a zone of inhibition of 23mm against Klebsilla pneumonia. The zone of inhibition by the plant extract was 11mm against Staphylococcus aureus, while the plant extract gel had a zone of inhibition of 16mm against Staphylococcus aureus. The results suggest that the bio-composite patches created from these materials have potential to work against both gram-positive and gramnegative bacteria. In a mouse model, the wound healing capacity was highest for the silver nanoparticle gel, with a wound closure of 1.7mm. Histological analysis also showed that the silver nanoparticle hydrogel had excellent wound repair properties. Overall, the study suggests that pure AgNPs and bio-composite hydrogel of AgNPs have efficient and quick healing of wounds as compared to the plant extract and plant nano composites hydrogel.