Abstract:
Pseudomonas aeruginosa is the main, fatal cause of infection in normal people as well
as in immunocompromised patients. The understanding of its genetic diversity and
phylogenetic is substantial for the epidemiological analysis and for management of
infections control. In order to study the genetic background of prevalent bacterium, 180
samples were isolated and out of these 180 samples, fourteen clinical isolates of
Pseudomonas aeruginosa was demonstrated for Multi-locus sequence typing and
comparative genomic analysis by utilizing whole genome sequences of these isolates
because these 14 strains are initially found with unknown sequence types. Further
analysis revealed the high genomic diversity of the strains and six out of fourteen
isolates were recognized as novel sequence types. GeoBURST analysis indicated
clones that are genetically similar to these new sequence types. The genomic analysis
confirmed the presence of determinants of antibiotics resistance and potential virulence
factors that might be helpful for targeted antibiotics therapy and designing of novel
vaccines based on anti-resistance against P. aeruginosa.
