Multi Locus Sequence Typing and Comparative Genomics of New Strains of Pseudomonas aeruginosa Isolated from Pakistan

Abstract

Pseudomonas aeruginosa is the main, fatal cause of infection in normal people as well as in immunocompromised patients. The understanding of its genetic diversity and phylogenetic is substantial for the epidemiological analysis and for management of infections control. In order to study the genetic background of prevalent bacterium, 180 samples were isolated and out of these 180 samples, fourteen clinical isolates of Pseudomonas aeruginosa was demonstrated for Multi-locus sequence typing and comparative genomic analysis by utilizing whole genome sequences of these isolates because these 14 strains are initially found with unknown sequence types. Further analysis revealed the high genomic diversity of the strains and six out of fourteen isolates were recognized as novel sequence types. GeoBURST analysis indicated clones that are genetically similar to these new sequence types. The genomic analysis confirmed the presence of determinants of antibiotics resistance and potential virulence factors that might be helpful for targeted antibiotics therapy and designing of novel vaccines based on anti-resistance against P. aeruginosa.