Investigating the anti-caner efficacy of Tetramethyulluteolin on MCF7 cell lines GCMS analysis A Metabolomics Approach

Abstract

Breast cancer is the most prevalent cancer in the globe, and Pakistan has the highest incidence and mortality of female breast cancer compared to all other cancer types. However, the current treatments for breast cancer are accompanied by a variety of side effects and limitations, including a weakened immune system, changes in hormonal levels, and an increase in the risk of various diseases. The current study focuses on investigating the anti-cancer efficacy of Tetramethylluteolin on MCF-7 cell lines using the In-silico and Invitro approaches. The gas chromatography-mass spectrometry (GCMS) technique was employed to identify metabolites with differential expression to understand Breast cancer metabolomics. Tetramethylluteolin is a flavonoid which possesses advantageous physiochemical properties with moderate lipophilicity and solubility with a higher absorption rate. In-silico and Invitro analysis revealed a stronger binding affinity with cancer-related proteins InterLeukin-6 and ILK, particularly in breast cancer pathways making it a potential therapeutic target for breast cancer pathways. In vivo investigations can validate the findings of this study and shed light on tetramethylluteolin's potential as a breast cancer treatment. Our research can guide the development of more effective and precisely targeted anti-cancer therapies.