Elucidating the Structure, Expression, along with the Prognostic Significance of KPCD in Breast Cancer

Abstract

Breast cancer is one of the most common and frequently occurring malignancy in women around the world, responsible for reported increase in death incidence. Both genetic and non genetic factors are involved in the onset and progression of breast cancer. KPCD belongs to novel KPC of the AGC kinases that plays a pivotal role in the development and progression of different cancers. A few studies have reported that KPCD can promote and suppress tumor progression depending on the interaction with its downstream signaling proteins. To date, there is no published data that define the correlation of KPCD with other genes in breast cancer progression. Therefore, the objective of the study is to evaluate the impact of KPCD protein protein interaction in cancer progression and to find the association of blood-based interaction of KPCD with clinical parameters. The study has reported the three-dimensional structure prediction and validation, evolutionary analysis, and conservational analysis of KPCD. Molecular dynamics analysis has revealed that the interactions between KPCD and SMAD3 for the mutated structure was less stable as compared to the native KPCD and SMAD3 interactions, which may contribute to the cancer progression. This study also evaluates the co expression of KPCD, TGF-B, and SMAD3 in the blood breast cancer patients. The study reported the elevated expression status of all three genes in breast cancer patients that may be serve as a potential biomarker for cancer diagnosis and treatment.