Evaluating Antibacterial And Wound Healing Activity Of Rumex acetosella Leaves

Abstract

Despite marvelous advances in the science and technology field, the significance of herbal remedies is irrefutable. The plant Rumex acetosella has been traditionally used as a wound-healing remedy for years. The objective of our study was to develop and evaluate a herbal product ensuring its biomedical application. In the current study, the herbal ointments were formulated using the pure and ethanolic extract of Rumex acetosella plant leaves. The ointment base was formulated using hard paraffin, soft paraffin, cetostearyl alcohol, and parabens as preservatives through the fusion method while the ointments were formulated by the incorporation of the drug into the base using the levigation method. The formulated ointments were subjected to different stability parameters such as physical appearance, temperature, pH, spreadability, LOD, washability, and non-irritancy tests. Moreover, the ointments were screened for having anti-bacterial potential against S.aureus and E.coli. All the formulations showed significant anti-bacterial efficacy against both stains but the ethanolic formulations were found to be more stable than pure extract formulations. All the formulations were acidic in nature, possessed notable spreadability, and less LOD. The patch test revealed that the formulations were non-irritant as they didn’t cause any allergic reaction or burning sensation to the skin. Among all the formulations, 7.5% w/w of ethanolic extract was more stable under extreme temperatures i.e., 4°C, 25°C, 37°C and above 40°C with a stable pH of 4.8.