Development of Ceftriaxone Containing Chitosan-Coated Catheters to Target Common Uropathogens for Preventing Catheter Associated Urinary Tract Infections

Abstract

Catheter Associated Urinary Tract Infections (CAUTIs) are a major healthcare concern and are linked to the use of indwelling urinary catheters. In this study, we present an approach for the prevention and treatment of CAUTIs by developing ceftriaxone-containing chitosan-coated catheters. Chitosan, a biocompatible and antimicrobial polymer, was utilized as a coating material for urinary catheters. Additionally, Ceftriaxone, a potent broad-spectrum antibiotic, was incorporated into the chitosan coating to establish a dual-action mechanism against UTI-causing pathogens. The uropathogens accumulate around the catheter surface and form biofilms which are sometimes very difficult to remove. The chitosan coating acts as a physical barrier, inhibiting bacterial adhesion and subsequent biofilm formation on the catheter surface. In vitro studies were conducted to assess the antimicrobial efficacy of ceftriaxone-containing chitosan-coated catheters against common uropathogens i.e., Staphylococcus aureus and Escherichia coli. Results of ceftriaxone containing chitosan-coated catheters in the form of Zone of Inhibition demonstrated better results as compared to uncoated catheters. Furthering up the research by taking in some other uropathogens and with further degradation, toxicity as well as surface morphological analyses effectivity of catheters can be enhanced.