Abstract:
The Trihelix transcription factors are referred as a GT element because of their binding capacity
with GT element. Trihelix transcription factors influence how plants grow and develop, as well
as how they respond to abiotic stresses. Although Trihelix transcriptions factors has described
and recognized in numerous plants, there is limited available knowledge regarding their presence
in Solanum tuberosum. The current investigation involved the characterization of trihelix genes
in Solanum tuberosum. Total of 27 StTH genes have been identified in Solanum tuberosum. All
of these 27 StTH were divided into five subgroups as previously described in Arabidopsis
thaliana (GT-2, GT-1, SH4, GT-y and SIP1). The genomic positioning and occurrences of gene
duplication revealed the arbitrary dispersion of StTH genes across 12 chromosomes, with
duplication events happening both in tandem and segmental patterns, involving 13 pairs of
genes. An analysis of synteny unveiled 19 orthologs of StTH genes within Solanum tuberosum
and 10 orthologs within Arabidopsis. Through in-silico promoter analysis, diverse arrays of cis elements and functional variations were discovered, with light-responsive and hormone responsive elements, along with development-related, abiotic stress-responsive, and promoter associated elements, prevailing in St Trihelix genes. In various biological and molecular
pathways, gene ontology unveiled the involvement of StTrihelix proteins, including defense
response, regulation of transcription, nucleotide transmembrane processes, ATP binding, and
phosphorylation. Two genes StTH 22 and StTH 25 were checked for drought stress. Both of
these gene’s show expression in stem, root and leaves, hence the more expression was analyzed
in leaves. This is the first study to look into how potato trihelix genes respond to drought stress.
The insights from our findings will be valuable for forthcoming research on the comparative and
molecular mechanisms of Trihelix genes in different plant species. To sum up, our discoveries
indicate the potential significance of StTH22 and StTH25 in contributing to essential roles under
drought stress conditions in potatoes.
