Abstract:
Acinetobacter baumannii, a bacterium classified as gram-negative, has become a significant
pathogen in hospital settings worldwide. It can cause a broad spectrum of illnesses and is of
particular concern because of its resistance to many antibiotics. Nevertheless, the investigation
of the genetic diversity and features of A. baumannii strains in specific geographical regions,
such as those obtained from Pakistan, has been rather limited. The present investigation
explores the genetic composition of A. baumannii strains by means of sequencing and
analyzing four genomes obtained from local sources. The research endeavors to elucidate the
genomic architecture by the utilization of comprehensive techniques such as genome
sequencing, assembly, and annotation. The following comparative study offers useful insights
into the genetic diversity that is inherent in the A. baumannii strains collected from the
surrounding area. The focus of this work is to analyze the presence of virulence factors and
antibiotic resistance determinants encoded within the genomes under study. The initial results
indicate a significant genetic diversity among the indigenous strains, thereby enhancing our
comprehension of the species' capacity to adapt and endure. The clinical significance of these
strains in hospital settings is underscored by the presence of genes linked to virulence factors
and antibiotic resistance mechanisms. Moreover, this research provides insight into the genetic
correlation between the strains collected locally and their global counterparts. The preliminary
phylogenetic analysis suggests the presence of genetically different lineages that are unique to
the strains found in Pakistan. This finding offers valuable information regarding the population
dynamics and evolutionary patterns of A. baumannii in this geographical area. In brief, this
study reveals the genomic composition of A. baumannii strains obtained from local sources,
revealing their genetic variability, possible virulence attributes, and patterns of antibiotic
resistance. Through the clarification of these elements, the research enhances the overall
comprehension of the clinical importance of A. baumannii and its consequences for healthcare
initiatives at the local level.
