Formation of Ciprofloxacin Loaded Transethosomes to Check the Antibacterial Activity Against Skin Infections

Abstract

One of the serious challenges in the treatment of infectious diseases is the presence of bacterial infections in subcutaneous wound tissue. Staphylococcus epidermidis (S. epidermidis) and Propionibacterium acne (P. acne) are resistant bacterial strains that cause severe disease in humans when penetrating the deeper layer of skin. Antibacterial drugs with a nonspecific target have more difficulty in penetrating the deeper layer of infected skin. Broad spectrum antibiotics are best to treat the infection but are not commonly used because bacteria’s make resistance against them. To overcome these issues, a combined strategy of broad-spectrum antibacterial drug and nanoparticle was formulated for targeted delivery, enhanced penetration to the infection site specifically. The ciprofloxacin was entrapped in transethosomes and formulation was synthesized by using the cold method. Transethosomes are very small in size that can reach the deeper layer of skin to give potential effects. In the layers of skin, ciprofloxacin works by binding to the enzymes topoisomerase IV and DNA gyrase and inhibit the DNA replication in bacteria. The antibacterial activity of ciprofloxacin loaded transethosomes against skin infections was assessed using Staphylococcus epidermidis and Propionibacterium acne and the method used was well diffusion method. The characterization of ciprofloxacin loaded transethosomes was done through, zeta potential, particle size evaluation, and drug release efficiency. Loaded transethosomes displayed substantially have more potential effect than ciprofloxacin alone. The in-vitro studies show that ciprofloxacin loaded transethosomes boost the antibacterial activity of ciprofloxacin against gram positive.