Evaluation of Control of MDR Campylobacter jejuni colonization by indigenous probiotics LAB in Broiler Chickens

Abstract

Campylobacteriosis is the most common zoonotic illness affecting people globally and contaminated chicken with Campylobacter jejuni, and is one of the major causes of enteric infections in humans. An efficient substitute i.e. the use of probiotics for lowering bacterial contamination in the livestock, which can strengthen the animals' natural defenses against harmful bacteria. In-vitro experiments showed that Lactobacillus reuteri and Enterococcus faecium isolated from the gut suppressed and control the development of C. jejuni count by using a 96-well microtiter plate and the plating technique. We designed an in vivo experiment using a poultry model to test the effectiveness of prospective indigenous probiotic strains against MDR Campylobacter jejuni in order to illustrate this impact in vivo. A poultry model was used to assess the effects of an indigenous probiotic combination of Enterococcus faecium and Lactobacillus reuteri on the growth performance of chicks and cecal microbiota of broiler chicks challenged with MDR Campylobacter jejuni. Total forty two, one-day-old birds were given an organic diet with probiotics (108 CFU/mL) in their drinking water, and they were given an oral challenge (105 CFU/mL) of Campylobacter jejuni using a micropipette. Probiotic administration reduced the cecal Campylobacter population (P<0.001) and considerably (P<0.01) increased feed efficiency and growth performance. Our in vivo study findings indicate that giving broiler chicks probiotics decreased the amount of C. jejuni cecal colonization.