Abstract:
The emergence of multidrug-resistant bacteria is a global health concern. Staphylococcus
aureus, particularly strains resistant to methicillin (MRSA), stands as a critical human pathogen
linked to both hospital and community-acquired infections. This study explored the potential of
Hermetia illucens (Black Soldier Fly) larval hemolymph as a natural antimicrobial source against
MRSA. This study investigated the impact of stress conditions, including physical injury and
MRSA exposure, on the antibacterial properties of BSF larval hemolymph. The larvae were
subjected to these stressors for varying durations, and the hemolymph was subsequently collected
from stressed as well as uninfected larvae for antibacterial testing against MRSA strains. The
results demonstrated a significant enhancement in the antibacterial activity of the hemolymph
under stress conditions, particularly after 24 hours of physical stress and 48 hours of bacterial
stress exposure. These findings suggest that stress-induced modulation in BSF larvae can
potentiate the antimicrobial efficacy of their hemolymph, providing valuable insights into natural
antimicrobial sources and their potential application in combating antibiotic-resistant infections.
