“Synthesis and characterization of Moringa coated Silk sutures for wound healing application

Abstract

Surgical sutures play a significant role during the wound healing of surgical site infection in the medical world. This study was done to develop novel Moringa Oleifera based polymeric composite films on the silk braided suture materials and produce an antimicrobial coating agent. Sodium hydroxide (0.1N) solution was used to pre-treat and open the structure of the sutures to allow the coating agent to penetrate the suture structure. Chitosan a natural polymer and Moringa Oleifera as an antibacterial drug are combined to get additional benefits such as prevention of surgical site infections (SSI) and wound healing. Moringa Oleifera extracts using in vitro antimicrobial screening methods. Chitosan and Moringa mixed in a fixed quantity to produce polymer-based films. The pretreated sutures were then evaluated and compared, with non-treated sutures acting as the control. The films were screened for antifungal and antibacterial activity against bacterial (P.aeruginosa, Escherichia coli) and fungal strains (Aspergillustubingensis and Aspergillusflavus) screened. Chitosan-based Moringa films showed antimicrobial activity against all the strains; the lowest Moringa concentration (5%) showed the highest activity against all the strains. In vitro antibacterial tests of these coated sutures were carried out. Through this study, it was found that the sutures coated with Moringa/Chitosan exerted a better-sustained efficacy assay compared to those that are not pretreated, have the potential to be used for wound healing.