Characterization of atrial natriuretic peptide (ANP) response in depression-induced mice models

Abstract

Depression is a complex psychological disorder that is also often link to the hormonal imbalances. Our research delves into the intricate relationship between depression and atrial Natriuretic Peptide (ANP), considering the multifaceted influences of neurological, genetic, and environmental factors. With a focus on hormonal imbalances as key contributors to depression, our investigation explores the potential therapeutic effects of Levothyroxine (LT4) in the context of ANP. Utilizing an early weaning mouse model involving maternal separation, we conducted a detailed examination of the anxiolytic effects of LT4, aiming to evaluate its efficacy in alleviating symptoms associated with anxiety and depression. Behavioral assessments and histological analyses were employed to comprehensively evaluate the impact of LT4 on ANP. This study also extends to molecular investigations using RT-PCR to analyze the distribution and expression of ANP within the mouse central nervous system, highlighting the cortex region. Our findings reveal significant differences in brain expression levels of ANP between treated mice and those exhibiting depressive symptoms. This insight suggests potential therapeutic applications of ANP for mitigating depression, presenting intriguing avenues for further research, particularly in the context of depression-induced mouse models through parental separation. This research contributes to an enriched understanding of the complex factors influencing depression and proposes interventions that extend beyond conventional approaches. The integration of behavioral assessments, histological analyses, and molecular investigations offers a holistic perspective, laying the groundwork for future exploration in the critical realm of mental health research.