Role of Schisandrin-A against Enterococcus faecalis in-vitro studies /

Abstract

This study delves into the investigation of Schisandrin-A's antibacterial efficacy against Enterococcus faecalis jh2-2, a pathogenic strain associated with escalating concerns of antibiotic resistance. Recognizing the pressing need for alternative treatments, we employed a multifaceted approach, including Minimum Inhibitory Concentration (MIC) testing, DNA extraction, Time-Kill Kinetics assays, and Scanning Electron Microscopy. Through meticulous MIC testing, concentration-dependent antibacterial effects of Schisandrin-A were revealed, showcasing its potential as a potent agent against Enterococcus faecalis. Drug DNA interaction techniques provided insights into the molecular dynamics of the compound's interaction, unraveling potential structural changes and binding modes with bacterial DNA. The Time-Kill Kinetics assays further elucidated the bactericidal activity of Schisandrin-A over varying concentrations and time intervals. These assays offered a comprehensive understanding of the compound's impact on bacterial viability, providing valuable data for assessing its therapeutic potential. Scanning Electron Microscopy unveiled morphological changes induced by SchisandrinA, shedding light on its mechanisms of action at a microscopic level. These findings collectively contribute to a nuanced comprehension of Schisandrin-A's antibacterial effects, offering insights into its potential application in combating antibiotic-resistant strains, particularly Enterococcus faecalis jh2-2. This research holds significant implications for public health, addressing the challenges posed by antibiotic-resistant bacterial strains. The identified antibacterial properties of Schisandrin-A provide a foundation for further exploration and development of alternative therapeutic strategies, aiming to mitigate the risks associated with conventional antibiotic treatments. Byvii unraveling the complex interplay between Schisandrin-A and Enterococcus faecalis jh2- 2, this study contributes to the ongoing efforts to confront the global issue of antibiotic resistance and enhance the arsenal of effective antibacterial agents.