Evaluating the role of KLF17 variant as a key regulator in Pathogenesis of Breast Cancer

Abstract

Breast cancer is one of the most prevalent cancer among women globally with higher metastatic potential and recurrence rate leading to greater mortality rates in females of Pakistan which demands for a promising prognostic biomarker of breast cancer to make early detection possible. KLF17 is a transcription factor and has been observed to play important role as tumor suppressor specifically inhibiting EMT but protein has received little consideration in perspective of Single Nucleotide Polymorphism. Therefore, the aim of this study was to analyze the pathogenic variants of KLF17 and its association with breast. The SNPs in KLF17 effect both the structure and function of KLF17 was investigated through in-silico analysis and the results observed were validated through the blood-based genotypic analysis via ARMS PCR. A positive association of C345R SNP was observed with pathogenesis of breast cancer, suggesting that rs183242786 could be used as a prognostic biomarker of Breast Cancer.