Abstract:
This research involves isolation and growth of bacteria from rotten apples and potatoes.
The whole genome sequencing revealed that Enterobacter mori was the cause of apples
and potatoes rotting. Genomic profiling and comparative genome analysis of E.mori was
carried out, which includes identification of antimicrobial resistance genes, virulence
genes, prophage regions, genomic islands and phylogenetic analysis. . The strain of
dictyospaerium DHSYM was used to extract algae oil, which was then utilized in the
production of algal oil emulsion. After that, this emulsion was incorporated into chitosan
hydrogel, and the chitosan hydrogel was coated over apples and potatoes to extend the shelf
life of these products and reduce the amount of post-harvest loss.
For characterizing, algal oil emulsion based chitosan hydrogel underwent a variety of
analyses. Such as scanning electron microscopy (SEM), Fourier transform infrared
spectroscopy (FTIR), Raman spectroscopy (RAMAN), testing for biofilm eradication,
antibiotic activity, hemolytic assay, cytotoxicity study, and molecular docking.
